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protocols for determining strain ploidy and plasmid copy number

Alpha1lab at aol.com Alpha1lab at aol.com
Mon Jan 17 18:43:41 EST 2000

I am looking for protocols for the following.  
1. Test for yeast strain ploidy, ie how do I verify a strain as being diploid 
vs. haploid?
2. Plasmid copy number  determination of 2 micron based vectors in S. 

Also, can haploid strains be transformed by standard tecniques, ie LiOAc?  
Can I maintain a full length 2 micron plasmid vector in a circle + strain by 
maintaining selective pressure?

3. What's a good protocol for curing endogenous 2 micron plasmid to make 
circle 0 strains?
Helen L. Gibson
alpha1lab at aol.com

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