IUBio Biosequences .. Software .. Molbio soft .. Network News .. FTP

About the extraction of total fatty acid in yeast

Kyung Man You kmy4 at cornell.edu
Fri Jan 14 15:44:06 EST 2000


Hi! Newsgroup,

I'm trying to extract total fatty acids in yeast Saccharomyces cerevisiae,
but I am not sure about my extraction procedure (The havested pellet is
washed with sterile water three times, spun briefly to remove as much water
as possible, and extracted with chloroform/methanol (2:1) at room temp. for
2 h. After solvent evaporation, the residue is treated with 0.5 M KOH in
methanol for 30 min, acidified with 1 M HCl, extract with hexane, and
concentrated under nitrogen. The resulting fatty acid methyl esters are
analyzed by GC/MS).

Can I get your comment or the exact procedure?

If you have any comment or new method about that, please let me know as soon
as possible.
Thank you!

-Kyung-
kmy4 at cornell.edu





------
Posted via news://freenews.netfront.net
Complaints to news at netfront.net
---





______________O_________oO_____________oO______o_______oO___________________
YEAST bionet newsgroup see: http://www.bio.net/hypermail/YEAST/
YEAST e-mail: messages sent to yeast at net.bio.net
subscribe: e-mail biosci-server at net.bio.net with: subscribe yeast
unsubscribe: e-mail biosci-server at net.bio.net with: unsubscribe yeast
YEAST on the WWW: http://genome-www.stanford.edu/Saccharomyces/VL-yeast.html
problems with the YEAST newsgroup? E-mail the moderator: francis at cmmt.ubc.ca






More information about the Yeast mailing list

Send comments to us at biosci-help [At] net.bio.net