Hallo,
I'm thinking about a yeast experiment that involves the genomic
integration of murine DHFR in an ORF with a relatively weak promoter.
Does somebody have experience with an experiment like that?
Can I successfully select for the stronger methotrexate resistance
(endogenous DHFR + transgene)?
Something like that wass obviously done for example by
>Gene 1986;50(1-3):225-37
>Construction of stable laboratory and industrial yeast strains
>expressing a foreign gene by integrative transformation using a
>dominant selection system.
>Zhu J, Contreras R, Fiers W
but they used a cytochrome C promoter and I couldn't find any
information on the strength of that.
Any hints, ideas?
Thank you, Johannes
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