> I have been using the pREP41-GFP vector which gives the insertan
> n-terminal GFP tag. This has worked sucessfully but I cannot find a
> vector map of the plasmid anywhere. Does anyone have a vector map or
> know where I can get one from?
I suspect there are several versions of these plasmids floating around.
The general map will be similar to REP41 (and REP3, etc:
see http://pingu.salk.edu/~forsburg/vectors.html for links to maps)
but for the details of the fusion, you'll have to figure out who made it
and how.
We have made a series of tagging vectors of modular design that can be
used to swap tags, plasmid markers, and different strengths of the
nmt promoter . However the only GFP version we have made
is full-strength nmt.
There is another set with different markers and linkers
from Tony carr and Iain Hagan, which sounds like the one you are using.
--
-susan
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S L Forsburg, PhD Associate Professor
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The Salk Institute, La Jolla CA
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Women in Biology Internet Launch Page
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