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a beast ate my yeast, help!

Ke Wei victorke at duke.edu
Fri Aug 11 11:51:55 EST 2000


I would try PCR on the BLUE colony.

Good luck!

On 1 Aug 2000, Dan Riggs wrote:

> Date: 1 AUG 2000 21:51:55 +0100 
> From: Dan Riggs <riggs at scar.utoronto.ca>
> Newgroups: bionet.molbio.yeast
> Subject: a beast ate my yeast, help! 
> 
> We have been conducting a protracted 2 hybrid screen and finally got a
> positive signal by x-gal selection.  Unfortunately, the master plate is
> contaminated by a fungus which grows much faster than does yeast (on
> triple drop out medium).  The yeast strain we are using is YRG2 from
> Stratagene, and its genotype doesn't suggest to me that one could expect
> to plate it on some selection medium to rid the cells of the
> contaminant.  Does anyone have any advice?
> 
> Thanks!
> 
> Dan Riggs
> 
> 
> 
> ---
> 
> 


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