I would try PCR on the BLUE colony.
On 1 Aug 2000, Dan Riggs wrote:
> Date: 1 AUG 2000 21:51:55 +0100
> From: Dan Riggs <riggs at scar.utoronto.ca>
> Newgroups: bionet.molbio.yeast
> Subject: a beast ate my yeast, help!
>> We have been conducting a protracted 2 hybrid screen and finally got a
> positive signal by x-gal selection. Unfortunately, the master plate is
> contaminated by a fungus which grows much faster than does yeast (on
> triple drop out medium). The yeast strain we are using is YRG2 from
> Stratagene, and its genotype doesn't suggest to me that one could expect
> to plate it on some selection medium to rid the cells of the
> contaminant. Does anyone have any advice?
>> Dan Riggs
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