Hello,
I have purified the DNA binding protein from yeast crude and/or
nuclear extract. Gel shift assay was performed as a marker of
activity. Although the predicted molecular mass of the protein
is 20-40 kDa, substantial amount of proteins, whose size are
60-80 kDa, were copurified during all of FPLC-purification proccess.
What are these proteins!
Please tell me about these suspicious proteins!
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HASHIGUCHI Kazunari
Ph.D Student, Laboratory of Radiation Biology,
Graduate School of Science, Kyoto University, JAPAN.
mailto:kaz at kingyo.zool.kyoto-u.ac.jp
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