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G418 selection

David Keszenman-Pereyra D.K-Pereyra at sheffield.ac.uk
Thu Apr 13 10:15:24 EST 2000

"can anyone tell me if it is possible to select for G418 resistant 
S.cerevisiae colonies on rich yeast medium containing 2% 
galactose and 1% raffinose? 
H. van Attikum"

We have plated yeast cells on SC-ura containing 2% galactose and 
G418 for integration of KNMX cassette and kept a URA3 plasmid.

David Keszenman-Pereyra
University of Sheffield
Department of Molecular Biology & Biotechnology   

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