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Southern blot with mtDNA?

Jukka Juselius Jukka.Juselius at vtt.fi
Thu Apr 6 00:01:06 EST 2000


I'm preparing a Southern blot to see if there are any deletions in the 
yeast mtDNA and wondering what would be a good probe for this experiment. I 
quess nuclearDNA contamination is a big problem in mtDNA Southerns if you 
don't first extract the mtDNA with CeCl gradient to really purify it from 
total DNA? I just thought to make minilysates (as described in Yeast, a 
practical approach, Ed. Campbell and Duffus) and do the Southerns with 
these. Suggestions would be highly appreciated!

Cheers,
Jukka

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