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Yeast media

Younes Bounhar ybounh at po-box.mcgill.ca
Wed Oct 27 00:11:36 EST 1999


I am trying to express proteins in yeast using a the Gal1 promoter) and
cannot afford to get raffinose (I tried glucose then galactose only and did
not get any induction. A friend of mine suggested to use a mixture of
3%Glycerol and 2%Ethanol. this allows for a very nice induction but the
cells grow very very slowly if at all (it takes over 72h to get an
OD600~0.6). Does anybody know why or if there is another alternative?

Younes

--
Be Yourself, no matter what they say





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