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constitutive expression in yeast

Christian Gauthier christian_gauthier at ircm.qc.ca
Fri Oct 15 16:26:46 EST 1999


Hi !
The plasmids you are seeking are the pRS423GPD (HIS3) and the pRS426GPD
(URA3). The GPD promoter is a very strong consitutive promoter. Those
plasmid are constructed on a pBluescript II SK backbone.
Christian Gauthier


Esbjorn Fiers wrote:
> 
> Does anybody know a plasmid for constitutive expression of a protein in
> yeast, with selection marker for yeast (prefer not LEU2 or TRP1) and
> bacteria.
> Thanks in advance,
> 
> Esbjorn Fiers
> 
> --
> ==================================================================
> Esbjorn Fiers
> DEPARTMENT OF GENETICS                         Fax:32 (0)9 2645349
> UNIVERSITY OF GENT, K. L. Ledeganckstraat 35, B-9000 Gent, Belgium
> Vlaams Instituut voor Biotechnologie                           VIB
> mailto:esfie at gengenp.rug.ac.be        http://www.plantgenetics.rug.ac.be
> 
> ==================================================================





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