Jo Evans <j.evans at har.mrc.ac.uk> wrote
> Can anyone recomend a protocol for isolating the 3 pombe
> chromosomes within an agarose plug (for use in pulsed-field gel
> electrophoresis) I'm using the method in Nurses fission yeast hand
> book at the moment but would like to play around with a few more
> methods.
We use this method quite a bit; our protocols are published in Liang et
al (1999), J. Cell Sci. 112:559-567 or Snaith and Forsburg, Genetics
152: 839-851.
--
-susan
:;:;:;:;:;:;:;:;:;:;:;:;:;:;:;:;:;:;
DON'T REPLY to the email address in header.
It's an anti-spam. Use the one below.
:;:;:;:;:;:;:;:;:;:;:;:;:;:;:;:;:;:;
S L Forsburg, PhD forsburg at salk.edu
Molecular Biology and Virology Lab
The Salk Institute, La Jolla CA
http://pingu.salk.edu/~forsburg/lab.html
Women in Biology Internet Launch Page
http://pingu.salk.edu/~forsburg/bio.html
:;:;:;:;:;:;:;:;:;:;:;:;:;:;:;:;:;:;
"These are my opinions. I don't have
time to speak for anyone else."
:;:;:;:;:;:;:;:;:;:;:;:;:;:;:;:;:;:;
______________O_________oO_____________oO______o_______oO___________________
YEAST bionet newsgroup see: http://www.bio.net/hypermail/YEAST/
YEAST e-mail: messages sent to yeast at net.bio.net
subscribe: e-mail biosci-server at net.bio.net with: subscribe yeast
unsubscribe: e-mail biosci-server at net.bio.net with: unsubscribe yeast
YEAST on the WWW: http://genome-www.stanford.edu/Saccharomyces/VL-yeast.html
problems with the YEAST newsgroup? E-mail the moderator: francis at cmmt.ubc.ca
