On 8 Nov 1999 21:10:11 -0800, S. Tan <sltan at u.washington.edu> wrote:
>Hey -
>>We've been doing routine yeast transformations in lab. Recently,
>however, we were unable to transform plasmids into our yeast strains (e.g.
>Hf7c from Clontech). We use fresh medium and new reagents, and have
>tried LiAc method and Bio101 kit. Does anyone know what might be going
>on? Do yeast cells lose "competency" after a long period of storage (at
>-80oC)?
In fact yeast cells lose their competence when you store it at -80°C
for a longer time period. I would never use such yeast cells for
transformation of a CDNA library for example. I allways would use
freshly prepaired cells for high efficiency transformations.
Regards
Mark
______________O_________oO_____________oO______o_______oO___________________
YEAST bionet newsgroup see: http://www.bio.net/hypermail/YEAST/
YEAST e-mail: messages sent to yeast at net.bio.net
subscribe: e-mail biosci-server at net.bio.net with: subscribe yeast
unsubscribe: e-mail biosci-server at net.bio.net with: unsubscribe yeast
YEAST on the WWW: http://genome-www.stanford.edu/Saccharomyces/VL-yeast.html
problems with the YEAST newsgroup? E-mail the moderator: francis at cmmt.ubc.ca
