Hey -
We've been doing routine yeast transformations in lab. Recently,
however, we were unable to transform plasmids into our yeast strains (e.g.
Hf7c from Clontech). We use fresh medium and new reagents, and have
tried LiAc method and Bio101 kit. Does anyone know what might be going
on? Do yeast cells lose "competency" after a long period of storage (at
-80oC)?
Thanks in advance,
Thomas
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