Dear Netters,
when doing two-hybrid screens with Clontech's S. cerevesiae HF7c
I occasionally observe some yeast colonies (around 10 per 10^6 cfu)
which have a really weird color (ranging from orange to bright
yellow - I'm not talking about the pink/red (ADE) phenotype...).
They grow like hell on triple dropout, they may have a somewhat
slimy appearance (yuk!) and they are often also LacZ positive.
I've never seen these guys in bait-only transfected cells though.
Just for the heck of it, I've tried to rescue some prey plasmids
but w/o much success.
Is anyone out there who has an idea what THAT is? Might be a
good source for a yellow pizza dough, though.
TIA
Hadi
Hadi Al-Hasani, Ph.D.
Center for Molecular Medicine
University of Cologne
Otto-Fischer-Str. 12-14
50674 Cologne
Germany
ph. +49.221.4706231
fax. +49.221.4705115
email. hadi.al-hasani.blahblah. at uni-koeln.de
