Just wanted to let you know that there is a real problem with the yeast
expression vectors available from ATCC. On at least two different
occasions, plasmids obtained from them had the wrong marker (e.g., his
rather than leu) and the wrong promoter (e.g., Gal rather than ADH). The
identity of what was sent was and is a mystery. Although ATCC recognizes
the problem and the fact that there have been many complaints, they are
unwilling to resolve this problem, again for reasons that are a mystery.
So, when ordering a yeast plasmid from ATCC, do not trust that you will
get what you thought you ordered.