I appologize if this sounds obnoxiuos, but are you sure you didn't knock
something important in your diploid with the Kan marker?
Hui Cai wrote:
> Hello All.
> I had a couple of quick questions, please, if anybody could help out.
>> 1) Is there a genetic background effect of G418 resistance using the
> NeoR gene?
> (I have a diploid strain with the resistance that grows fine on
> YPD+G418, but,
> after I sporulate none of the spores will grow on YPD+G418)
>> 2) Possibly just a historical question, but, why does it seem that
> antibiotic resistance
> markers are used so seldomly in yeast work?
>> Thanks for your help!
>hcai at ucla.edu
Krassen Dimitrov, Ph.D.
Department of Molecular Biotechnology,
University of Washington,
Seattle, WA 98195
phone: (206) 685-6892
e-mail: krasen at u.washington.edu