I had a couple of quick questions, please, if anybody could help out.
1) Is there a genetic background effect of G418 resistance using the
(I have a diploid strain with the resistance that grows fine on
after I sporulate none of the spores will grow on YPD+G418)
2) Possibly just a historical question, but, why does it seem that
markers are used so seldomly in yeast work?
Thanks for your help!
hcai at ucla.edu