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(Fwd) Re: Determination of live yeast

Hui Cai hcai at ucla.edu
Tue Mar 16 11:46:46 EST 1999


Not sure if this would work for you...  the FUN-1 Dye will give different
fluorescence based on if the
cell is alive or not...  (red v. green).  This method requires a FACS type
device.  I think the dye is made by Molecular Probes.

Also, you could try Methylene Blue (I think I use .025% solution as a 10x
dye, I might be off by another factor of 10).  Live cells will reduce the
dye, dead cells will not.  So, 1 ul in 10ul of cells and then look at them
under a microscope
and count...

> ------------
> > To:            nobody at net.bio.net
> > From:          Ken Hazen <krhazen at aloha.net>
> > Subject:       Determination of live yeast
> > Date:          Sat, 06 Mar 1999 07:59:50 -1000
> > Hello,
> > I would like to determine how much of a yeast culture is active or
> > alive. I am going to conduct a trial to determine the benefits of
> > feeding active yeast on animal performance. Not only do I want to learn
> > if their is a benefit to feeding yeast to animals, but does it have to
> > be active to show the response.  Is there a simple test to determine
> > amount of active (live) yeast in a feed grade yeast product?
> > Thank you in advance for any help you can give me.
> > Ken Hazen
> >
> >
> >
> >

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