Not sure if this would work for you... the FUN-1 Dye will give different
fluorescence based on if the
cell is alive or not... (red v. green). This method requires a FACS type
device. I think the dye is made by Molecular Probes.
Also, you could try Methylene Blue (I think I use .025% solution as a 10x
dye, I might be off by another factor of 10). Live cells will reduce the
dye, dead cells will not. So, 1 ul in 10ul of cells and then look at them
under a microscope
> > To: nobody at net.bio.net> > From: Ken Hazen <krhazen at aloha.net>
> > Subject: Determination of live yeast
> > Date: Sat, 06 Mar 1999 07:59:50 -1000
>> > Hello,
> > I would like to determine how much of a yeast culture is active or
> > alive. I am going to conduct a trial to determine the benefits of
> > feeding active yeast on animal performance. Not only do I want to learn
> > if their is a benefit to feeding yeast to animals, but does it have to
> > be active to show the response. Is there a simple test to determine
> > amount of active (live) yeast in a feed grade yeast product?
> > Thank you in advance for any help you can give me.
> > Ken Hazen