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yeast 2 hybrid - NO POSITIVES

Elizabeth Vallen evallen1 at swarthmore.edu
Thu Mar 11 17:29:48 EST 1999

We have been using Phil James' 2hybrid library and yeast strain (described
in a Genetics paper about 1-2 years ago).  It sounded great in the paper.
The bait plasmid is a low copy TRP-based GAL4 DNA binding domain, the
library a LEU-based GAL4 DNA activation domain and the strain has three
different markers which report on two-hybrid activity.  We have screened
the library with 3 different baits and have not found any true positives
(we get out some positives, but none are plasmid-linked).  I was wondering
if anyone had had good luck with this library and system.  What GAL4
-activation domain libraries have been successful for you?   Please reply
by email if possible.

Thanks for your help,

Liz Vallen
Assistant Professor
Department of Biology
500 College Ave
Swarthmore College
Swarthmore, PA 19081

on leave 1998-99
(215) 573-6677

(610) 328-8048
(610) 328-8663 (FAX)

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