We have been using Phil James' 2hybrid library and yeast strain (described
in a Genetics paper about 1-2 years ago). It sounded great in the paper.
The bait plasmid is a low copy TRP-based GAL4 DNA binding domain, the
library a LEU-based GAL4 DNA activation domain and the strain has three
different markers which report on two-hybrid activity. We have screened
the library with 3 different baits and have not found any true positives
(we get out some positives, but none are plasmid-linked). I was wondering
if anyone had had good luck with this library and system. What GAL4
-activation domain libraries have been successful for you? Please reply
by email if possible.
Thanks for your help,
Department of Biology
500 College Ave
Swarthmore, PA 19081
on leave 1998-99
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