I wondered if anyone would have some information (precise or anecdotal)
on the homologous recombination frequencies in S.cerevisiae for the
purposes of gene replacement or plasmid integration. In my experience I
get about the same number of recombinant clones as the number of
transformants I get with a mere plasmid transformation. Does that mean
that once the DNA piece gets in the cell it recombines with a frequency
of ~1.00 or that my plasmid transformation is less efficient than my
linear DNA transformation?
Thank you very much for your cooperation!
Krassen Dimitrov, Ph.D.
Department of Molecular Biotechnology,
University of Washington,
Seattle, WA 98195
phone: (206) 685-6892
e-mail: krasen at u.washington.edu