I need a yeast expression plasmid with a lys2 selection marker and a
constitutive or gal induced promoter as I have run out of markers in the
strain that I MUST use. Could anyone out there help me?
I was also considering an alternative: the strain I have to use is
ADE2. I have good plasmids to express genes with an ADE2 marker, but I
would have to make the strain ade2. I could mate, sporulate etc, but I
was wondering if I transform with an ade2 gene fragment and plate
onto YPD plates, is the rate of homologous recombination such that I
would be abe to pick out pink colonies ?
Your advice is gratefully anticipated!
Scott Vande Pol
sbv at po.cwru.edu