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buffering media

Bernard P. Murray, PhD bpmurray*STUFFER* at socrates.cgl.ucsf.EDU
Mon Mar 1 17:30:53 EST 1999

In article <7b6l8u$r7d at net.bio.net>, Jason Plotkin
<plotkij at rockvax.rockefeller.edu> wrote:

> I am trying to prepare synthetic complete media containing 0.1 M phosphate
> buffer pH=7.0. When I add phosphate to YNB/ammonium sulfate/ ura dropout
> powder solution a precipitate slowly forms.
> I have been able to prepare this solution in the past (at least I think so)
> and do not know what's wrong now. Does anyone have any experience with
> buffering yeast medias? What could be going wrong?
> Sincerely,
> Jason

What counter-ion are you using for the phosphate buffer?
If its Na+ then maybe try K+.
     Just a thought,
Bernard P. Murray, PhD
Dept. Cell. Mol. Pharmacol., UCSF, San Francisco, USA

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