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Yeast Promoters??

news at OHSU.EDU news at OHSU.EDU
Thu Jun 17 01:06:41 EST 1999


Hi,

I would look at the following articles:

Ronicke et al. Methods in Enzymology (1997) 283: 313-322.
Belli et al. Nucleic Acids Research (1998) 26: 942-947.

Mike

In article <7k805m$5nr at net.bio.net>, daemon at hgmp.mrc.ac.uk, Michelle
Bausch <bausch at rsbs.anu.edu.au> wrote:

> Hi,
> 
> I will soon be screening a cDNA library in a yeast expression vector.  I
> wish to use either a constitutive low/weak yeast promoter, or an inducible
> promoter as I would like to control the level of expressed proteins to
> prevent  toxicity effects.  Does anyone recommend any particular yeast
> promoter except GAL promoters, and Cu inducible??  Has anyone had any
> experience with the acid phosphatase promoter (PHO5)?  Are there any other
> suggestions?  Any reply will be much appreciated!!
> 
> Many thanks,
> 
> Michelle
> ---
> 



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