I would look at the following articles:
Ronicke et al. Methods in Enzymology (1997) 283: 313-322.
Belli et al. Nucleic Acids Research (1998) 26: 942-947.
In article <7k805m$5nr at net.bio.net>, daemon at hgmp.mrc.ac.uk, Michelle
Bausch <bausch at rsbs.anu.edu.au> wrote:
>> I will soon be screening a cDNA library in a yeast expression vector. I
> wish to use either a constitutive low/weak yeast promoter, or an inducible
> promoter as I would like to control the level of expressed proteins to
> prevent toxicity effects. Does anyone recommend any particular yeast
> promoter except GAL promoters, and Cu inducible?? Has anyone had any
> experience with the acid phosphatase promoter (PHO5)? Are there any other
> suggestions? Any reply will be much appreciated!!
>> Many thanks,
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