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Yeast Promoters??

daemon at hgmp.mrc.ac.uk daemon at hgmp.mrc.ac.uk
Wed Jun 16 06:01:10 EST 1999


I will soon be screening a cDNA library in a yeast expression vector.  I
wish to use either a constitutive low/weak yeast promoter, or an inducible
promoter as I would like to control the level of expressed proteins to
prevent  toxicity effects.  Does anyone recommend any particular yeast
promoter except GAL promoters, and Cu inducible??  Has anyone had any
experience with the acid phosphatase promoter (PHO5)?  Are there any other
suggestions?  Any reply will be much appreciated!!

Many thanks,


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