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colony lifts

biosci-request at net.bio.net biosci-request at net.bio.net
Fri Jun 11 03:07:01 EST 1999


Much obliged!!  

Has anyone out there used this protocol with much success?  If there are
any "tricks" that you want to share, please feel free to do so!

Thanks again!
peace
Gina

On 10 Jun 1999 biosci-request at net.bio.net wrote:

> Hey Gina,
> 	Check out a protocol in "Methods in Yeast Genetics: A Laboratory
> Course Manual" by Mark Rose, Fred Winston, and Phil Hieter.  Technique "3:
> Yeast Colony Hybridization.   I bet you can get this in the library, or
> one of the yeast labs.
> 	Or try Grunstein and Hogness, PNAS 72: 3961-1965 (1975).
> 
> On Wed, 9 Jun 1999 biosci-request at net.bio.net wrote:
> 
> > 
> > Hi All--
> > 
> > Does anyone have a protocol/methodology for doing yeast colony lifts? I'm
> > looking for a quick way to screen a few hundred colonies for a
> > yes/no answer without going through the standard genomic DNA isolation for
> > all these clones.  
> > 
> > Any and all suggestions would be appreciated.
> > 
> > Thanks in advance!
> > 
> > Gina Alvino
> > alvino at u.washington.edu
> > 
> > 
> > 
> > There's nothing quite like the sound of a Harley Davidson.....
> > 
> > 
> > 
> > 
> --------------------------------------------------------------------------
>          @..@             Rick Stevens
>         (\--/)            Department of Pathology
>        (.>__<.)           Box 357705
>        ^^^  ^^^           University of Washington
>    ''''''''''''''''       Seattle, WA  98195-7705
> --------------------------------------------------------------------------
> 



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