I used 5-FOA plates that were about 6 month old without problem. I grew
the cells overnight in YPD, resuspended them in water, and dropped 5-10ul
on the plate(SD-whatever for selecting the other plasmids, + 5-FOA) .
However, my experience was with 2u plamids.
Dept. of Biochemistry
Univ. of Wisconsin-Madison
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