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5-FOA plate stability/useful life

news at news.doit.wisc.edu news at news.doit.wisc.edu
Mon Jun 7 22:55:57 EST 1999


I used 5-FOA plates that were about 6 month old without problem.  I grew
the cells overnight in YPD, resuspended them in water, and dropped 5-10ul
on the plate(SD-whatever for selecting the other plasmids, + 5-FOA) . 
However, my experience was with 2u plamids.

Beilin Zhang
Dept. of Biochemistry
Univ. of Wisconsin-Madison



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