I am performing a two-hybrid screening (Gal4, Matchmaker Two-Hybrid
System 2) using a protease inhibitor as bait and a chondrocyte cDNA
library as prey.
About 1/3 of my positive clones are proteins with LIM-Domains (mainly
DRAL and zyxin).
Does anyone have experiences with LIM-Domains in two-hybrid screens ?
Are they known false positives ?
Same with cytochrome b-245 ? I also found some of those !
Please give me a hint !
Thanks in advance !
biomedpage at hotbot.comhttp://come.to/biomedpage
Sent via Deja.com http://www.deja.com/
Share what you know. Learn what you don't.