> From: Carl Blobel (c_blobel at ski.mskcc.org)
> Hello everyone.
>> In screening a S. pombe cDNA library, I have isolated several clones
> which contain an ORF I am interested in. However, they are all
> downstream of a second ORF. Based on a comparison with known genomic
> sequences, the cDNAs have been properly spliced and do not represent the
> ligation of two seperate cDNAs. I have been unable to find any
> published reports of polycistronic mRNAs and was wondering if anyone has
> had any similar observations. Thanks!
>> Johannes Schlondorff
>
What is the size of your upstream ORF?
It is not uncommon for pombe to have extremely long upstream UTRs, often
with multiple small ORFs in them. This seems to be especially common in
genes induced by starvation. Whether this implicates translational
control a la GCN4, has yet to be determined.
-susan
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