Hello everyone.
In screening a S. pombe cDNA library, I have isolated several clones
which contain an ORF I am interested in. However, they are all
downstream of a second ORF. Based on a comparison with known genomic
sequences, the cDNAs have been properly spliced and do not represent the
ligation of two seperate cDNAs. I have been unable to find any
published reports of polycistronic mRNAs and was wondering if anyone has
had any similar observations. Thanks!
Johannes Schlondorff
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