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Polycistronic pombe mRNA

Carl Blobel c_blobel at ski.mskcc.org
Tue Dec 28 13:05:05 EST 1999


Hello everyone.

In screening a S. pombe cDNA library, I have isolated several clones
which contain an ORF I am interested in.  However, they are all
downstream of a second ORF.  Based on a comparison with known genomic
sequences, the cDNAs have been properly spliced and do not represent the
ligation of two seperate cDNAs.  I have been unable to find any
published reports of polycistronic mRNAs and was wondering if anyone has
had any similar observations. Thanks!

Johannes Schlondorff

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