IUBio

intracellular volume determination

Ron Lahav ronil at bgumail.bgu.ac.il
Wed Dec 15 02:38:04 EST 1999


In order to determine intracellular volume a protocol using tritiated water
and an "impermeable" substance, usually [14C]inulin is used.
Does anyone know whether it matters if the inulin is Inulin-[14C]carboxylic
acid or Inulin-[14C]methoxy. The price difference is substantial and so I
would like to order the cheapest one. Also if anyone has had experience
using a different substance, other than Inulin I would appreciate hearing.
Thanks again.




*********************************************

Ron Lahav, Ph.D. student
Environmental Hydrology & Microbiology
Jacob Blaustein Institute for Desert Research
Sde Boker Campus
Ben Gurion University of the Negev.
Beer Sheva, Israel.

Lab telephone: -972-7-659-6836
Lab fax: -972-7-659-6831


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