Greetings everyone,
I was wondering if anyone has any references to work that examined the
numbers and types of mutations induced by different concentrations of EMS
for S. cerevisiae. I am looking for a simple protocol that will generate a
small number of "random" mutations in a particular strain. I have thought
of Ty mutagenesis as well, but our lab is very new and we are still waiting
on delivery of our thermocycler and other molecular type machines.
Thanks in advance for your time.
Happy Holidays,
Scott
------------------------------------------------------------------------
Scott D. Pletcher
Max Planck Institute for Demographic Research
Doberaner Strasse 114
D-18057 Rostock, Germany
Phone: (+49) 381 2081 164
Fax: (+49) 381 2081 464
email: pletcher at demogr.mpg.de
-----------------------------------------------------------------------
______________O_________oO_____________oO______o_______oO___________________
YEAST bionet newsgroup see: http://www.bio.net/hypermail/YEAST/
YEAST e-mail: messages sent to yeast at net.bio.net
subscribe: e-mail biosci-server at net.bio.net with: subscribe yeast
unsubscribe: e-mail biosci-server at net.bio.net with: unsubscribe yeast
YEAST on the WWW: http://genome-www.stanford.edu/Saccharomyces/VL-yeast.html
problems with the YEAST newsgroup? E-mail the moderator: francis at cmmt.ubc.ca
