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Metabolic labeling of yeast proteins

buhler buhler at jonas.saclay.cea.fr
Thu Dec 9 05:09:36 EST 1999


In reply to Jason query,
I bet you will find a thourough answer for yeast in vivo labelling  in
the materials and methods paper :
Rapid identification of yeast proteins on two-dimensional gels.
       Maillet I, Lagniel G, Perrot M, Boucherie H, Labarre J
       J Biol Chem 1996 Apr 26 271:17 10263-70
abstract
This work describes a rapid and sensitive technique for the
identification of Saccharomyces cerevisiae proteins on two-dimensional
 gels based on the determination of their amino acid ratios. Specific
double labeling with 3H and 14C or 35S-labeled amino acids,
 chosen among those that are specifically incorporated into proteins
without interconversion, ...

Good luck!

Jean-Marie BUHLER
SBGM/DBCM, Bat. 142,
Centre d’Etude Nucléaire Saclay,
91191, Gif sur Yvette,
France
phone: 33 1 69 08 64 18
fax:      33 1 69 08 47 12






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