IUBio Biosequences .. Software .. Molbio soft .. Network News .. FTP

Yeast medium

Byung-Hoon Kim byung-hoon.kim at uni-tuebingen.de
Thu Dec 2 15:36:49 EST 1999

Hi everyone,

I have a question about yeast medium and yeast growth.
Using a galatose inducible S. cerevisiae expression system., I am trying
to coexpress two plant proteins.
The standard medium would be <Yeast Nitrogen Base, 2% Galactose, and
Systhetic Drop out for selection>
But I couldn't get the expression (at the level of western analysis) of
the two proteins at the same time.

I could get the coexpressed proteins when I reduced the galactose
concentration to 0.5%.
(In addition to 0.5% galactose, 1.5% Glycerol was added.)

The question is whether this medium could have a negative effect for
normal physiological state in yeast.
Because one of my proteins is stress regulatory protein, I'm afraid that
the modified medium triggers a stress reaction and interfere my futher
experiment. Of course I think the coexpression of foreign proteins
itself is a kind of stress, but I'd like to exclude any other possible
stress factors.

I appricate any comment.

Byung-Hoon Kim
Center for Plant Molecular Biology
Univ. Tuebingen

YEAST bionet newsgroup see: http://www.bio.net/hypermail/YEAST/
YEAST e-mail: messages sent to yeast at net.bio.net
subscribe: e-mail biosci-server at net.bio.net with: subscribe yeast
unsubscribe: e-mail biosci-server at net.bio.net with: unsubscribe yeast
YEAST on the WWW: http://genome-www.stanford.edu/Saccharomyces/VL-yeast.html
problems with the YEAST newsgroup? E-mail the moderator: francis at cmmt.ubc.ca

More information about the Yeast mailing list

Send comments to us at biosci-help [At] net.bio.net