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mimimal medium for ade2- ade3- strain ?

biosci-request at net.bio.net biosci-request at net.bio.net
Tue Aug 31 10:28:08 EST 1999


>Subject: mimimal medium for ade2- ade3- strain ?>>>Hi to all,>>I make transformation of his3- ade2- ade3- by His3+ plasmid. What is the>minimal selective medium for the transformant?>Thanks,>Ilya

The ADE3 gene product is needed for both adenine and for histidine
biosynthesis. Thus, ade3 mutants are also His- auxotrophs. 

David Stillman
stillman at hci.utah.edu





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