IUBio Biosequences .. Software .. Molbio soft .. Network News .. FTP

yeast growth

biosci-request at net.bio.net biosci-request at net.bio.net
Tue Aug 31 08:37:49 EST 1999


biosci-request at net.bio.net, David Humair wrote:
> 
> Hi netters,
> I have some problems with yeast growth.
> People say: grow yeast overnight! OK! My question is how long is
> overnight!
> I'm working with minimal inducing medium (yeast are shifting from
> glucose to galactose). I start culture with on OD600=0.4. After 16
> hours, OD600=0.54=1.16x10E6 cells/ml. Is it normal? Normally, we need
> 1-2x10E7. How long it will take to reach this level, in minimal inducing
> medium?
> 
> Best regards
> 
> David Humair

Hey David,
at which temperature? Some yeast strains need under minimal conditions
temperatures up to 35°C and more than only 16h. Start your culture in
the morning and you will get  a good output on the next morning. Gently
shaking could help, too.
Greeting
Carsten Kettner




______________O_________oO_____________oO______o_______oO___________________
YEAST bionet newsgroup see: http://www.bio.net/hypermail/YEAST/
YEAST e-mail: messages sent to yeast at net.bio.net
subscribe: e-mail biosci-server at net.bio.net with: subscribe yeast
unsubscribe: e-mail biosci-server at net.bio.net with: unsubscribe yeast
YEAST on the WWW: http://genome-www.stanford.edu/Saccharomyces/VL-yeast.html
problems with the YEAST newsgroup? E-mail the moderator: francis at cmmt.ubc.ca




More information about the Yeast mailing list

Send comments to us at biosci-help [At] net.bio.net