biosci-request at net.bio.net, David Humair wrote:
>> Hi netters,
> I have some problems with yeast growth.
> People say: grow yeast overnight! OK! My question is how long is
> overnight!
> I'm working with minimal inducing medium (yeast are shifting from
> glucose to galactose). I start culture with on OD600=0.4. After 16
> hours, OD600=0.54=1.16x10E6 cells/ml. Is it normal? Normally, we need
> 1-2x10E7. How long it will take to reach this level, in minimal inducing
> medium?
>> Best regards
>> David Humair
Hey David,
at which temperature? Some yeast strains need under minimal conditions
temperatures up to 35°C and more than only 16h. Start your culture in
the morning and you will get a good output on the next morning. Gently
shaking could help, too.
Greeting
Carsten Kettner
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