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yeast growth

biosci-request at net.bio.net biosci-request at net.bio.net
Mon Aug 30 09:01:03 EST 1999


Hi netters,
I have some problems with yeast growth.
People say: grow yeast overnight! OK! My question is how long is
overnight!
I'm working with minimal inducing medium (yeast are shifting from
glucose to galactose). I start culture with on OD600=0.4. After 16
hours, OD600=0.54=1.16x10E6 cells/ml. Is it normal? Normally, we need
1-2x10E7. How long it will take to reach this level, in minimal inducing
medium?

Best regards

David Humair

--

David Humair
Laboratoire de biochimie
Emile-Argand 9
Case Postale 2
CH-2007 Neuchbtel 7

Tel: +41327182226
Fax: +41327182231
Email: David.Humair at bota.unine.ch

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"Tell a man there are 400 billion stars and he'll believe you;
tell him a bench has wet paint and he has to touch it."
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