Hi netters,
I have some problems with yeast growth.
People say: grow yeast overnight! OK! My question is how long is
overnight!
I'm working with minimal inducing medium (yeast are shifting from
glucose to galactose). I start culture with on OD600=0.4. After 16
hours, OD600=0.54=1.16x10E6 cells/ml. Is it normal? Normally, we need
1-2x10E7. How long it will take to reach this level, in minimal inducing
medium?
Best regards
David Humair
--
David Humair
Laboratoire de biochimie
Emile-Argand 9
Case Postale 2
CH-2007 Neuchbtel 7
Tel: +41327182226
Fax: +41327182231
Email: David.Humair at bota.unine.ch
----------
"Tell a man there are 400 billion stars and he'll believe you;
tell him a bench has wet paint and he has to touch it."
----------
______________O_________oO_____________oO______o_______oO___________________
YEAST bionet newsgroup see: http://www.bio.net/hypermail/YEAST/
YEAST e-mail: messages sent to yeast at net.bio.net
subscribe: e-mail biosci-server at net.bio.net with: subscribe yeast
unsubscribe: e-mail biosci-server at net.bio.net with: unsubscribe yeast
YEAST on the WWW: http://genome-www.stanford.edu/Saccharomyces/VL-yeast.html
problems with the YEAST newsgroup? E-mail the moderator: francis at cmmt.ubc.ca
