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Protein expression in yeast

biosci-request at net.bio.net biosci-request at net.bio.net
Sat Aug 28 17:05:31 EST 1999


Hi all,

I cloned several genes in the p42x-gal1 vectors and transformed YPH499 S.
cerevisiae. Although the transformation yielded several colonies on
selective medium...none of the ones I tested seem to express anything after
16 or 24h. I also tried different protein prep methods.
Does anyone have any idea?

Best regards,

Younes

--
Be Yourself, no matter what they say





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