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electroporation of s.cerevisiae?

biosci-request at net.bio.net biosci-request at net.bio.net
Fri Aug 27 05:47:51 EST 1999


Try and use  a lower voltage (1.5 kV). Check your time constant (should be
between 4 and 5). I am also having some pbs with electroporation
however...not very consistent.
Try this web page:
http://www.umanitoba.ca/faculties/medicine/units/human_genetics/gietz/Trafo.html

Younes
<biosci-request at net.bio.net> wrote in message news:7pilj6$9ej at net.bio.net...
> Dear All,
>
> I have been working with S.pombe for a while,using a number of
> transformation methods and usually do electroporation.I have started
> working with cerevisiae and have tried the same conditions (1700v,
> 200ohms, 25uF) on cells resuspended in 1M sorbitol using 2mm
> electroporation cuvettes. It has not been as successful as for pombe
> and was wondering if anyone has a quick method for cerevisiae
> electroporation.
>
> Kindest regards
>
> Nick
> nicholas.owen at anat.oxford.ac.uk
>





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