IUBio Biosequences .. Software .. Molbio soft .. Network News .. FTP

Electroporation: yeast DNA > E.coli

biosci-request at net.bio.net biosci-request at net.bio.net
Wed Aug 18 20:02:57 EST 1999


Is there a specific reason for using electroporation?  There are other
methodologies that may work better.

> 
> <biosci-request at net.bio.net> wrote in message news:7p1fd8$3ft at net.bio.net...
> > Hi everyone,
> > i am trying to transfer plasmid DNA from yeast to E.coli by
> > electroporation. I followed
> > the protocol  from Nucleic Acids Research Vol.20 N4, 1992 with no
> > success. Searching
> > on the net  I also didn´t find anyone who managed.
> > Do you know a new protocol / new conditions / etc that i could follow?
> > Thanks for your answer.
> >
> > E-mail: nuria.costa-roldan at gpc-ag.com






______________O_________oO_____________oO______o_______oO___________________
YEAST bionet newsgroup see: http://www.bio.net/hypermail/YEAST/
YEAST e-mail: messages sent to yeast at net.bio.net
subscribe: e-mail biosci-server at net.bio.net with: subscribe yeast
unsubscribe: e-mail biosci-server at net.bio.net with: unsubscribe yeast
YEAST on the WWW: http://genome-www.stanford.edu/Saccharomyces/VL-yeast.html
problems with the YEAST newsgroup? E-mail the moderator: francis at cmmt.ubc.ca




More information about the Yeast mailing list

Send comments to us at biosci-help [At] net.bio.net