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Electroporation: yeast DNA > E.coli

biosci-request at net.bio.net biosci-request at net.bio.net
Wed Aug 18 20:02:57 EST 1999

Is there a specific reason for using electroporation?  There are other
methodologies that may work better.

> <biosci-request at net.bio.net> wrote in message news:7p1fd8$3ft at net.bio.net...
> > Hi everyone,
> > i am trying to transfer plasmid DNA from yeast to E.coli by
> > electroporation. I followed
> > the protocol  from Nucleic Acids Research Vol.20 N4, 1992 with no
> > success. Searching
> > on the net  I also didn´t find anyone who managed.
> > Do you know a new protocol / new conditions / etc that i could follow?
> > Thanks for your answer.
> >
> > E-mail: nuria.costa-roldan at gpc-ag.com

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