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Electroporation: yeast DNA > E.coli

news at piglet.cc.uic.edu news at piglet.cc.uic.edu
Wed Aug 18 13:38:51 EST 1999


Could you be more specific, what plasmid, what contains, what conditions

<biosci-request at net.bio.net> wrote in message news:7p1fd8$3ft at net.bio.net...
> Hi everyone,
> i am trying to transfer plasmid DNA from yeast to E.coli by
> electroporation. I followed
> the protocol  from Nucleic Acids Research Vol.20 N4, 1992 with no
> success. Searching
> on the net  I also didn´t find anyone who managed.
> Do you know a new protocol / new conditions / etc that i could follow?
> Thanks for your answer.
>
> E-mail: nuria.costa-roldan at gpc-ag.com






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