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S. cerevisiae Ino- plates...

biosci-request at net.bio.net biosci-request at net.bio.net
Tue Aug 17 19:58:39 EST 1999


Hi all,

I am interested in studying the ino1 promoter, and need to make ino-
media to check for this auxotrophy.  I understand that Difco makes a
vitamin free nitrogen base, which does not contain inositol, but does
contain glucose (unlike YNB - amino acids, which most people use).
Since I am also interested in using different carbon sources aside from
glucose, I was wondering if anyone knew of a either a yeast nitrogen
base that I could use, or a pre-mix (like bio101 or clonetech) which was
ino- and -carbon source...
of course, as a last resort I am willing to make the media from
scratch...in which case I was wondering if anyone had suggestions as to
what vendor would be good for these supplies (I understand our lab uses
sigma for most of our amino acids....and difco for the agar/nitrogen
base).

I'd appreciate any thoughts/recipes/vendors you could share...thanks in
advance!

My email is valmik at alum.mit.edu

-valmik





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