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Electroporation: yeast DNA > E.coli

biosci-request at net.bio.net biosci-request at net.bio.net
Fri Aug 13 10:59:36 EST 1999

Hi everyone,
i am trying to transfer plasmid DNA from yeast to E.coli by
electroporation. I followed 
the protocol  from Nucleic Acids Research Vol.20 N4, 1992 with no
success. Searching 
on the net  I also didn´t find anyone who managed. 
Do you know a new protocol / new conditions / etc that i could follow?
Thanks for your answer.

E-mail: nuria.costa-roldan at gpc-ag.com

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