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ORF knockout question

news at newsreader.wustl.edu news at newsreader.wustl.edu
Tue Aug 10 16:44:55 EST 1999


I would like to generate a null allele of my new favorite gene in yeast.
The gene I'm interested in is very close to the neighboring gene, and they
are oriented in opposite directions, such that there is only 234 bases
between the start codons.  I'm concerned that the promoter of the
neighboring gene extends into the coding sequence of the gene I want to
knock out.  If this is true, if I knock out the whole coding sequence, I
will also disturb normal expression of the neighboring gene.

With the tightly packed yeast genome, this must be a relatively common
problem, how should I deal with it?

Thanks for any input,

Alex Brands

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