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In article <7oodda$pku at net.bio.net>, biosci-request at net.bio.net, wrote:
>> I have just begun a project and would like to heterologously express a
> cDNA clone in S.pombe. Does anyone know of a tight, inducible promoter
> for S.pombe other than the CaMV coexpressed tet repressor or nmt (which
> is too leaky)?
>> ---
> Luke Carrangis
It's actually a de-repressible promoter, but the nmt1 promoter works very
well. Simply switch to thiamine-free medium, and within a few cell cycles
you get very good levels of expression. The plasmid pESP-1 is what we use
and it works very well.
Colin Rasmussen
U of Saskatchewan
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