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an inducible promoter for S.pombe

news at tribune.usask.ca news at tribune.usask.ca
Tue Aug 10 14:03:46 EST 1999



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In article <7oodda$pku at net.bio.net>, biosci-request at net.bio.net, wrote:


>
> I have just begun a project and would like to heterologously express a
> cDNA clone in S.pombe. Does anyone know of a tight, inducible promoter
> for S.pombe other than the CaMV coexpressed tet repressor or nmt (which
> is too leaky)?
>
> ---
> Luke Carrangis

It's actually a de-repressible promoter, but the nmt1 promoter works very
well.  Simply switch to thiamine-free medium, and within a few cell cycles
you get very good levels of expression.  The plasmid pESP-1 is what we use
and it works very well.

Colin Rasmussen
U of Saskatchewan




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