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pombe inducible promoters

biosci-request at net.bio.net biosci-request at net.bio.net
Tue Aug 10 13:09:55 EST 1999




> Message-Id: <199908100530.WAA26273 at net.bio.net>
> 
> To: yeast at net.bio.net
> 
> Date: Tue, 10 Aug 1999 14:49:53 +0930 (CST)
> 
> From: biosci-request at net.bio.net
> 
> Subject: an inducible promoter for S.pombe
> 
> I have just begun a project and would like to heterologously express a
> cDNA clone in S.pombe. Does anyone know of a tight, inducible promoter
> for S.pombe other than the CaMV coexpressed tet repressor or nmt (which
> is too leaky)?
> 

The short answer is there is as yet no such promoter in general circulation.

The longer answer is that there is a newly reported promoter, the INV
promoter 
(see recent paper by Russell's lab).  However, it is not clear whether
this is 
any less leaky than nmt1 and it cannot be used for sustained expression.
 Note that the
leakiness of nmt is a function of the stability of the protein;  for
unstable proteins
it provides a tight shutoff.



-susan
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