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ubiquitin-mediated conditional alleles in S. pombe

biosci-request at net.bio.net biosci-request at net.bio.net
Sun Aug 8 18:57:43 EST 1999


Has ubiquitation-mediated protein destabilization been used to make
conditional mutations in S. pombe?  My laboratory would like to use this
to shut down the expression of an allele of an essential gene which when
under the control of the weak nmt1 promoter, is still too high to cause
a growth defect even in the presence of thiamin.

--
Richard J. Maraia, M.D.
Chief, Section on Molecular and Cell Biology
Laboratory of Molecular Growth Regulation
National Institute of Child Health and Human Development
National Institutes of Health
6/416  MSC-2753
Bethesda, MD 20892
Phone 301 402-3567
Fax: 301 480-6863
Email: maraiar at mail.nih.gov






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