Has ubiquitation-mediated protein destabilization been used to make
conditional mutations in S. pombe? My laboratory would like to use this
to shut down the expression of an allele of an essential gene which when
under the control of the weak nmt1 promoter, is still too high to cause
a growth defect even in the presence of thiamin.
--
Richard J. Maraia, M.D.
Chief, Section on Molecular and Cell Biology
Laboratory of Molecular Growth Regulation
National Institute of Child Health and Human Development
National Institutes of Health
6/416 MSC-2753
Bethesda, MD 20892
Phone 301 402-3567
Fax: 301 480-6863
Email: maraiar at mail.nih.gov
______________O_________oO_____________oO______o_______oO___________________
YEAST bionet newsgroup see: http://www.bio.net/hypermail/YEAST/
YEAST e-mail: messages sent to yeast at net.bio.net
subscribe: e-mail biosci-server at net.bio.net with: subscribe yeast
unsubscribe: e-mail biosci-server at net.bio.net with: unsubscribe yeast
YEAST on the WWW: http://genome-www.stanford.edu/Saccharomyces/VL-yeast.html
problems with the YEAST newsgroup? E-mail the moderator: francis at cmmt.ubc.ca
