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Media pH

biosci-request at net.bio.net biosci-request at net.bio.net
Sun Aug 8 18:57:11 EST 1999


I tried to use the citrate/phosphate buffer protocol from the Cold Spring 
harbor manual to make YNB media pH 2 to 6 or 7.  When I titrated the 
citrate/phosphate (1M/2M, pH2.5) to pH 3 with solid KOH, I was okay.  When 
pH reached 4, I got precipitate.  What else can I do to make YNB media 
buffered pH range from 2 to 7?  Thanks


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