IUBio Biosequences .. Software .. Molbio soft .. Network News .. FTP

Cloning multiple genes on a vector

biosci-request at net.bio.net biosci-request at net.bio.net
Wed Aug 4 10:59:40 EST 1999


Has anyone had any experience with cloning multiple (2-4) genes into a
Saccharomyces vector?  Are there problems with repeating promoter and
terminator sequences, and are there any sources for vectors containing
2 or more promoter-terminator cassettes?

Any info/advice would be greatly appreciated!

Thanks

Robyn Roth
Research Scientist, Biotechnology Programme
CSIR Division of Bio/Chemical Technologies
Private Bag X2
1645 Modderfontein
South Africa
tel:  +27-11-605 2264
fax:  +27-11-608 3020
e-mail:  rroth at csir.co.za





______________O_________oO_____________oO______o_______oO___________________
YEAST bionet newsgroup see: http://www.bio.net/hypermail/YEAST/
YEAST e-mail: messages sent to yeast at net.bio.net
subscribe: e-mail biosci-server at net.bio.net with: subscribe yeast
unsubscribe: e-mail biosci-server at net.bio.net with: unsubscribe yeast
YEAST on the WWW: http://genome-www.stanford.edu/Saccharomyces/VL-yeast.html
problems with the YEAST newsgroup? E-mail the moderator: francis at cmmt.ubc.ca
                                                                      
                                                                      
                                                                      
                                                                      
                                                                      
                                                                      
                                                                      
                                                                      
                                                                      
                                                                      
                                                                      
                                                                      
                                                                      
                                                                      
                                                                      
                                                              




More information about the Yeast mailing list

Send comments to us at biosci-help [At] net.bio.net