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[adenine] to obtain white ade2- strain?

news at news.chorus.net news at news.chorus.net
Wed Aug 4 10:55:27 EST 1999

On 3 Aug 1999 00:07:20 -0700,
    biosci-request at net.bio.net, ilya ilya (ilya_il at yahoo.com) wrote:
>I'm growing S. cerevisiae up to stationary phase (OD600 about 6). The
>red color 
>produced by the ade2- strain interferes with OD measurement. Are
>anybody known how 
>many adenine should I add to YPD medium to obtain "white" cell culture?

I don't think that adding adenine will help -- at least it does
not in my experience.  Use an ade2, ade3 strain instead.

Ashok Aiyar, Ph.D.
McArdle Laboratory for Cancer Research
aiyar at ebv.oncology.wisc.edu

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