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[adenine] to obtain white ade2- strain?

news at mlucom4.urz.uni-halle.de news at mlucom4.urz.uni-halle.de
Wed Aug 4 10:54:19 EST 1999


biosci-request at net.bio.net, ilya ilya wrote:
> 
> Hi,
> I'm growing S. cerevisiae up to stationary phase (OD600 about 6). The
> red color
> produced by the ade2- strain interferes with OD measurement. Are
> anybody known how
> many adenine should I add to YPD medium to obtain "white" cell culture?
> 
> Thanks in advance
> ilya
> _____________________________________________________________
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Hi,

we use 100 mg/L, but it doesn't work in all cases. So far we
could  not figure out why the cells still turn red sometimes.

Ricky

ATGCATCGTGATCAATCGTATCCCGTGGAAGTACGTACGTAGCACGG

Frederik Boernke
Research Group of  Molecular Plant Physiology
Institute for Plant Genetics and Crop Plant Research (IPK)
Corrensstr. 3
06466 Gatersleben
Tel.  039482 -5 321
Fax. 039482 -5 515
http://www.ipk-gatersleben.de






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